Investigation of some physiological, biochemica characteristics and multiple antibiotic resistance of Staphylococcus aureus isolated from wound samples

Authors

  • Hasanain Ahmed Enad Obaid Department of Biology, Graduate School of Natural and Applied Sciences, Çankiri Karatekin University, Cankiri, Turkey

Keywords:

Staphylococcus aureus, antibiotic resistance, biochemical properties, burns and wounds

Abstract

In this study, carried out at Baghdad University, it was aimed to determine the optimum antibiotics that will affect Staphylococcus aureus infection and live contaminated microorganisms, which cause different disease problems in Iraqi patients who have been exposed to various burns and injuries. For this purpose, about 100 samples were collected from these patients, who were in different age ranges and had various injuries and burn wounds, and were transferred to different culture media. As a result of the growths occurring in the environment, 60% of S. aureusbacteria and 40% of other bacterial species were detected. Specimens contaminated with S. aureusbacteria showed high resistance to the antibiotic tobramycin (56.7%), and less resistance to Gentamaisin (30%) and Imikacin (10%). Bacterial isolators showed a negative effect on the endol reaction and a positive effect on the oxidis reaction in the filling culture medium. Next, two methods were applied to examine the ability of bacteria to form a thin biofilm. The first is the red congou medium method, which measures the ability of bacteria to form an adhesive layer. In the second method, micro-titer plate method (MTP) were used to measure the bacteria's ability to form the sticky layer. As a result, all insulators showed very low adhesive formation in the method using calibration cups.

References

Al-Asady FM, Al-Saray DA, Obed AW. Incidence of septicemia. Etiology and antimicrobial susceptibility testing among patients admitted to tertiary care hospital. The Journal of Infection in Developing Countries. 2020;14(12):1387-1394.

Aklilu E, Zunita Z, Hassan L, Cheng CH. Molecular epidemiology of methicillin- resistant Staphylococcus aureus (MRSA) among veterinary students and personnel at a veterinary hospital in Malaysia.Veterinary Microbiology. 2013;164(3-4):352-358.

Al-Taee EH, Al-Naimi RA, Znad KH, Al-Tamimi AA. Study the Histopathological Changes and Bacteriological Causes of Natural Infection of the Livers in Sheep at Diyala Province. Diyala Journal for Pure Science, 2017, 13(4-part 1).

AL-Tikrity TAH, Al-Douri MTA, Abdul-Aziz MM, Al-Jebouri UMA. Diagnostic Values of Complete Blood Count in Patients with Urinary Tract Infection. Indian Journal of Public Health Research & Development, 2019, 10(10).

Al-Dahmoshi HOM. Genotypic and phenotypic investigation of alginate biofilm formation among Pseudomonas aeruginosa isolated from burn victims in Babylon, Iraq. Science Journal of Microbiology, 2013.

Bronowskil C, James CE, Winstanley C. Role of environmental survival in transmission of Campylobacter jejuni. FEMS Microbiol. Lett. 2014;356(1):8-19.

Babapour E, Haddadi A, Mirnejad R, Angaji SA, Amirmozafari N. Biofilm formation in clinical isolates of nosocomial Acinetobacter baumannii and its relationship with multidrug resistance. Asian Pac. J. Trop. Biomed. 2016;6(6):528-533.

Basset P, Feil EJ, Zanetti G, Blanc DS. 25-The Evolution and Dynamics of Methicillin-Resistant Staphylococcus aureus. In M. Tibayrenc (Ed.), Genetics and Evolutionof Infectious Disease, 2011, 669-688.

Collee JG, Fraser AG, Marmion BP, Simmons A. Practical medical microbiology. 14th ed. Longman. Singapore Publishers (pet) ltd. Singapore, 1996, 363-373.

Cruickshank R, Duguid JP, Marmion BP, Swain RHA. Medical Microbiology. 12th ed Churchill. Livingstone (publ.), London, 1975.

Chuang YY, Huang YC. Molecular epidemiology of community-associated meticillin-resistant Staphylococcus aureus in Asia. The Lancet Infectious Diseases. 2013;13(8):698-708.

Den Heijer CDJ, Van Bijnen EME, Paget WJ, Pringle M, Goossens H, Bruggeman CA, et al. Prevalence and resistance of commensal Staphylococcus aureus, including meticillin-resistant S aureus, in nine European countries: a cross-sectionalstudy. The Lancet Infectious Diseases. 2013;13(5):409-415.

Forbes BA, Schaberg DR. Transfer of resistance plasmids from Staphylococcus epidermidis to Staphylococcus aureus: evidence for conjugative exchange of resistance. Journal of Bacteriology. 2007;153(2):627-634.

Freeman D, Falkiner F, Keane C. New method for detecting slime production by coagulase-negative staphylococci. J. Clin. Pathol. 1989;42:872-874.

Hou W, Sun X, Wang Z, Zhang Y. Biofilm-forming capacity of Staphylococcus epidermis’s, Staphylococcus aureus, and Pseudomonas aeruginosa from ocular infections. Investigative Ophthalmol. Visual Sci. 2012;53(9):5624-5631.

Hotterbeekx A, Kumar-Singh S, Goossens H, Malhotra-Kumar S. In vivo and In vitro Interactions between Pseudomonas aeruginosa and Staphylococcus spp. J. Front. Cell. Infect. Microbiol. 2017;7:106.

Hong JS, Yoon E-J, Lee H, Jeong SH, Lee K. Clonal Dissemination of Pseudomonas aeruginosa sequence type 235 isolates carrying bla IMP-6 and emergence of bla GES-24 and blaIMP-10 on novel genomic islands PAGI-15 and -16 in South Korea. Antimicrob. Agents Chemother. 2016;60:7216-7223.

Hashim I. Microbiology culture media in pharmaceutical industry, 2013.

Jevons MP. “Celbenin”-resistant staphylococci. British Medical Journal. 1961;1(5219):124.

Holden MTG, Feil EJ, Lindsay JA, Peacock SJ, Day NPJ, Enright MC, et al. Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance. Proceedings of the National Academy of Sciences of the United States of America. 2004;101(26):9786-9791.

Karthi R, Selvachandra M, Jamuna Devi P. Perspective of insurance agents' about LIC of India-A study at Nagapattinam District. In IEEE-International Conference on Advances in Engineering, Science And Management (ICAESM), 2012, 63-69.

Khan MA, Faiz A. Antimicrobial resistance patterns of Pseudomonas aeruginosa in tertiary care hospitals of Makah and Jeddah. Ann. Saudi Med. 2016;36(1):23-28.

Giridhar G, Kreger AS, Myrvik QN, Gristina AG. Inhibition of Staphylococcus adherence to biomaterials by extracellular slime of S. epidermidis RP12. Journal of biomedical materials research. 1994;28(11):1289-1294.

LaSarre B, Federle MJ. Exploiting quorum sensing to confuse bacterial pathogens. Microbiol. Mol. Biol. Rev. 2013;77:73-111.

Luzzago C, Locatelli C, Franco A, Scaccabarozzi L, Gualdi V, Vigano R, et al. Clonal diversity, virulence-associated genes and antimicrobial resistance profile of Staphylococcus aureus isolates from nasal cavities and soft tissue infections in wild ruminants in Italian Alps. Veterinary Microbiology. 2014;170(1):157-61.

Murray PR, Baron EJ, Sen JH, Landry PM. Manual of Clinical Microbiology. 9th ed (9th ed). American Society for Microbiology, 2007.

Mathur T, Singhal S, Khan S, Upadhyay DJ, Fatma T, Rattan A. Detection of biofilm formation among the clinical isolates of staphylococci: an evaluation of three different screening methods. Indian journal of medical microbiology. 2006;24(1):25-29.

MacFaddin FJ. Biochemical Tests for Identification of Medical Bacteria. 3rd ed. The Williams and Wilkins Baltimore. U.S.A, 2000.

Purrello SM, Daum RS, Edwards GFS, Lina G, Lindsay J, Peters G, et al. Meticillin-resistant Staphylococcus aureus (MRSA) update: New insights into bacterial adaptation and therapeutic targets. Journal of Global Antimicrobial Resistance. 2012;2(2):61-69.

Noto MJ, Kreiswirth BN, Monk AB, Archer GL. Gene acquisition at the insertion site for SCCmec, the genomic island conferring methicillin resistance in Staphylococcusaureus. Journal of Bacteriology. 2008;190(4):1276-1283.

Stringfellow K, Anderson P, Caldwell D, Lee J, Byrd J, McReynolds J. Evaluation of disinfectants commonly used by the commercial poultry industry under simulated field conditions. Poultry science. 2009;88(6):1151-1155.

Sekar M, Jaffar FNA, Zahari NH, Mokhtar N, Zulkifli NA, Kamaruzaman RA, et al. Comparative evaluation of antimicrobial properties of red and yellow rambutan fruit peel extracts. Annual Research & Review in Biology, 2014, 3869-3874.

Selim S, Elkholy I, Hagagy N, ElAlfay S, Abedl Aziz M. Rapid identification of Pseudomonas aeruginosa by pulsed - field gel electrophoresis. Biotechnol. Biotechnol. Equipment. 2015;1(29):152-156.

Shulman JA, Nahmias AJ. Staphylococcal infections; clinical aspects. In: The Staphylococci. Edited by Cohen, J O, 1972, 457-481.

Senior BW, Albrechtsen M, Kerr M. A survey of IgA protease production among clinical isolates of the proteases. J. Med. Microbiol. 1988;25:27-31.

Saleha A, Zunita Z. Methicillin resistant Staphylococcus aureus (MRSA): An emerging veterinary and zoonotic pathogen of public health concern and some studies in Malaysia. Journal Animal Veterinary Advances. 2010;9(7):1094-1098.

Tashiro Y, Yawata Y, Toyofuku M, Uchiyama H, Nomura N. Interspecies interaction between Pseudomonas aeruginosa and other microorganisms. J. Microbes Environ. 2013;28:13-24.

Tadesse A, Alem M. Medical bacteriology. University of Gondar, 2006, 61-63.

Tille P. Bailey and S cotts Diagnostic Microbiology –E- Book: Elsevier Health Sciences, 2014.

Tille P. Bailey and Scotts Diagnostic Microbiology–E- Book: Elsevier Health Sciences, 2015.

Velkov T, Roberts KD, Nation RL, Thompson PE, Li J. Pharmacology of polymyxins: new insights into an ‘old’ class of antibiotics. Future microbiology. 2013;8(6):711-724.

Vandepitte J, Verhaegen J, Engbaek K, Rohner P, Piot P, Heuck CC. Basic laboratory procedures in clinical Bacteriology. 2nd.World Health Organization Geneva, 2003, 109-120.

Wiles TJ, Kulesus RR, Mulvey MA. Origins and virulence mechanisms of uropathogenic Escherichia coli. Exper. Molec. Pathol. 2008;85(1):11-19.

Zunita Z, Bashir A, Hafizal A. Occurrence of Multidrug Resistant Staphylococcus aureus in horses in Malaysia. Veterinary World. 2008;1(6):165-167.

Downloads

Published

2023-05-11

How to Cite

Obaid, H. A. E. (2023). Investigation of some physiological, biochemica characteristics and multiple antibiotic resistance of Staphylococcus aureus isolated from wound samples. Journal of Advanced Education and Sciences, 3(3), 09–16. Retrieved from https://www.dzarc.com/education/article/view/329

Issue

Section

Articles