Contraceptive utility of jequirity bean-Abrus precatorious (Rosary pea/Gunja) in the male rats

Abstract

The dosage and administration of 50% Ethanolic Extract of Jequirity Bean Seeds extract at 0’75gm/kg (75mg/kg) dosage for 45 and 90 days. The Male Rat Groups Treated and Control were administered after 24 hrs of last dose under Di-ethyl ether or Chloroform Anesthesia and dissected immediately. Reproductive Organs of Rats like Testis and Epididymis were taken out, freed from adherent tissue and blood. The weight of both Organs was recorded separately. The significance difference of weight between the treated and control rats was assessed by student, unpaired 't' test taking P<0'05 as load of significance. The Testis and Epididymis was fixed in Bowin's fluid, washed, dehydrated and embedded in Paraffin wax. The Tissues were sectioned at 5 micron and stained with stain of Haemotoxyle & Eocin for Histological studies purpose. After dissection and makes permanent histological slides, we found various types of cells in the Seminiferous tubules which were normal in structure but Spermatogonia and Spermatocytes displayed a few changes in their structures. The initial and final body weight of the experimental and control group rats were recorded for measures their differences if appears.